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Cell cycle analysis of Chinese hamster ovary (CHO) cells; BrdU detection using DNase ; BrdU detection by antibody Protocol; DNase Protocol ; Publications. BrdU is light sensitive and should be added in the dark. Supplies.
:�A� %��������� x�\���>����f�ngG�l��`r���|H���N$�~��F�ͮ}jw�V�����O����X��L9��������_��_L�旲���� ��ڶ|���u�:[][�c��m��0M�gwoʾgX��{[���0Qy�C�ײ�������M����te���]m (?�+��ZDD �����wk�훺{��>�k�c3dp)..C�`�Qg������|��Q�aܨ3j�Xs��Z�\͵uw:�k⠪j$]���L�SOaRY�fp�]< �cg�d��)����B3���0S*ެ��6m��$ʢ�2�=#� The BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells by flow cytometric analysis. [V��o�~s��o6�w���;&=%ɵ���vT��7o��B�{�OAF�6��NCݕ���U. Cycling cells are incubated with 5-bromo-2’- deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.Please use caution when handling. 1X Phosphate Buffered Saline (PBS): Dissolve 8 g NaCl, 0.2 g KCl, 1.44 g Na 2 HPO 4 and 0.24 g KH 2 PO 4 in 800 mL distilled water (dH 2 O).
X�KC?��;nY����NP�[ޢ� ��D���I �R%�� '��������m�q��0t3E������i�.E�i�x5�F;d�OІ ? Specific For: BrdU (Bu20a) Mouse mAb #5292. The BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells by flow cytometric analysis. Rɛ���궅Ƚ}��ćQd{��{���ءv��C���T� o}��b�����������B�'qö*2��� �"���˃��D�2H�c^�S�[��!c ��(m롬L��/6�1+[�, !��ƏD� ���)d]l=�� MlU�N�'TX���Q�#�=bA��E����_,�^.������M����E�Gƒm��7!$JjY'Ԕ�W �6��� � ߺq����oo]��C�_6���s����\�����/ѹF��^��Y�Bk��|���wڰ(з�`�켨�ۨ�2��l��%L�7�6�@�V�bp�K����{�L�(�ս� /tmD!��Uo6��fl'��~*!�QʌT��î�4��@B����b�I��-��`�O���W��/�x�ޠ�E8� .
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%PDF-1.3 Cycling cells are incubated with 5-bromo-2’- deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. A. Y؇��Zt�#]rId����9`��D��٪tA�W���D}���C����"Q>t��7���x��U�t)CI�(C�( ҵ�A6n�(��d���>x �C�D��ȷ�Jb��+�0�*�$�Dx$ �r+R�C!�t�K�����W�º�4�"p�H0��kt(%���&���ԟ�D��j@���n1���aa#l��wE�Ѣ��e�f� _��o��J��j��s�Dl�c�z}�� H �?�ۆ�.��.��"]f��c��$?�_LA۹�.D�=� ;֯���Ȧ�������$��F�]�a 낅A�rvdqAh��Ѧ���}��\3��6�|!��|]GO�Վ�����8y�@4��?�mRX?W��d�=�&d�.A#h�'���j��~���7N@������y%�d� Flow Cytometry BrdU Modified Protocol. Adjust the pH to 7.4 with HCl and the volume to 1 liter. Cells pulsed with BrdU may be photosensitive — incubations should be in the dark as well. ���l,�6�[ �,jzN��$� ��E��#�Cd�C|�j���A:f�Wۦ;@��FD��D�!X�(�c���� Yale School of Medicine Immunobiology; Flow Cytometry Facility; BrdU Staining Protocol . 2) Cells with no BrdU pulse, and only the antibodies added. Yale Flow Cytometry. Depending on the cell type, the BrdU pulse can be as short as 6 min and still be detectable by flow cytometry.